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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
31/03/2021 |
Actualizado : |
31/03/2021 |
Tipo de producción científica : |
Trabajos en Congresos/Conferencias |
Autor : |
GOLDBERG, V.; MACEDO, F.; CIAPPESONI, G. |
Afiliación : |
VIRGINIA GOLDBERG BIANCHI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FERNANDO LIBER MACEDO, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay; CARLOS GABRIEL CIAPPESONI SCARONE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
SNP genotyping for parentage identification in a Merino nucleus and in a commercial Highlander flock. |
Complemento del título : |
Volume Electronic Poster Session - Genetic Gain - Genotyping & Phenotyping Strategies, , 359. |
Fecha de publicación : |
2018 |
Fuente / Imprenta : |
In: Proceedings of the World Congress on Genetics Applied to Livestock Production, 11., Aotea Centre Auckland, New Zealand: WCGALP, ICAR, 11-16 feb 2018, 359. |
Idioma : |
Inglés |
Contenido : |
ABSTRACT.
Pedigree information is required to estimate breeding values accurately and to ensure high rates of genetic gain. DNA markers information can be used as an alternative to traditional recording of pedigree, being SNPs the markers of choice for parentage verification. The aim of the present study was to determine sheep parentage by SNPs using a very low density panel in: 1) a stud Merino flock to know the percentage of parentage error in order to correct pedigree misidentification and; 2) a commercial Highlander flock, to study the possibility to not control lambing anymore because for the breeder it is very laborious, time-consuming and disturb the relationship ewe-lambs during parturition. Genomic DNA was isolated from 200 samples of Merino sheep in 2015 and from 108 and 904 samples of Merino and Highlander sheep in 2016, respectively; and were genotyped with a very low density panel containing 507 SNPs. In 2015, for the 91 lambs genotyped, the error in parentage assignment was 16.5%. Although the assigned sire did not match with the declared sire for 15 lambs, the true sire was assigned for 14 of them. Thus, 99% of the lambs genotyped, had a sire assigned by the SNP panel. For the 80 lambs with their dams genotyped, the error rate was 12.5%. For the 10 lambs with mismatches with the declared dams, the true dam was assigned for five of them. In 2016, Merino samples were genotyped to link only lambs with their sires. For the 101 lambs, the error of parentage assignment was 21.8%. For Highlander, 7.5% of samples failed the genotyping and analyses was conducted without knowledge of the relationships between lambs with sires and dams. The 51% of the lambs genotyped had a sire and a dam assigned, 21% had only the sire assigned and 15% had only assigned a dam. Thus, genotyping by SNPs assigned a sire to the 72% of the lambs and a dam to the 66% of the lambs. The main problem was that only 5 of the 11 rams used as sires were genotyped and the high percentage of samples which failed the genotyping. However, taking into account the lack of information related to pedigree, we consider that a high rate of lambs had a sire and a dam assigned by the SNP panel. In conclusion, the development of a SNP panel for parentage assignment at a low price, would provide breeders with the opportunity of making mating management and control lambing easier and more relaxed while improving the known of pedigree information, ensuring high rates of genetic gain. Keywords: molecular markers, parentage exclusion, parentage verification, sheep. MenosABSTRACT.
Pedigree information is required to estimate breeding values accurately and to ensure high rates of genetic gain. DNA markers information can be used as an alternative to traditional recording of pedigree, being SNPs the markers of choice for parentage verification. The aim of the present study was to determine sheep parentage by SNPs using a very low density panel in: 1) a stud Merino flock to know the percentage of parentage error in order to correct pedigree misidentification and; 2) a commercial Highlander flock, to study the possibility to not control lambing anymore because for the breeder it is very laborious, time-consuming and disturb the relationship ewe-lambs during parturition. Genomic DNA was isolated from 200 samples of Merino sheep in 2015 and from 108 and 904 samples of Merino and Highlander sheep in 2016, respectively; and were genotyped with a very low density panel containing 507 SNPs. In 2015, for the 91 lambs genotyped, the error in parentage assignment was 16.5%. Although the assigned sire did not match with the declared sire for 15 lambs, the true sire was assigned for 14 of them. Thus, 99% of the lambs genotyped, had a sire assigned by the SNP panel. For the 80 lambs with their dams genotyped, the error rate was 12.5%. For the 10 lambs with mismatches with the declared dams, the true dam was assigned for five of them. In 2016, Merino samples were genotyped to link only lambs with their sires. For the 101 lambs, the error of parentage assignm... Presentar Todo |
Palabras claves : |
Molecular markers; Parentage exclusion; Parentage verification; Sheep. |
Thesagro : |
MARCADORES MOLECULARES; OVINOS. |
Asunto categoría : |
L10 Genética y mejoramiento animal |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/15439/1/Goldberg-et-al.-2018.-WCGALP.pdf
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Marc : |
LEADER 03308nam a2200205 a 4500 001 1061916 005 2021-03-31 008 2018 bl uuuu u01u1 u #d 100 1 $aGOLDBERG, V. 245 $aSNP genotyping for parentage identification in a Merino nucleus and in a commercial Highlander flock.$h[electronic resource] 260 $aIn: Proceedings of the World Congress on Genetics Applied to Livestock Production, 11., Aotea Centre Auckland, New Zealand: WCGALP, ICAR, 11-16 feb 2018, 359.$c2018 520 $aABSTRACT. Pedigree information is required to estimate breeding values accurately and to ensure high rates of genetic gain. DNA markers information can be used as an alternative to traditional recording of pedigree, being SNPs the markers of choice for parentage verification. The aim of the present study was to determine sheep parentage by SNPs using a very low density panel in: 1) a stud Merino flock to know the percentage of parentage error in order to correct pedigree misidentification and; 2) a commercial Highlander flock, to study the possibility to not control lambing anymore because for the breeder it is very laborious, time-consuming and disturb the relationship ewe-lambs during parturition. Genomic DNA was isolated from 200 samples of Merino sheep in 2015 and from 108 and 904 samples of Merino and Highlander sheep in 2016, respectively; and were genotyped with a very low density panel containing 507 SNPs. In 2015, for the 91 lambs genotyped, the error in parentage assignment was 16.5%. Although the assigned sire did not match with the declared sire for 15 lambs, the true sire was assigned for 14 of them. Thus, 99% of the lambs genotyped, had a sire assigned by the SNP panel. For the 80 lambs with their dams genotyped, the error rate was 12.5%. For the 10 lambs with mismatches with the declared dams, the true dam was assigned for five of them. In 2016, Merino samples were genotyped to link only lambs with their sires. For the 101 lambs, the error of parentage assignment was 21.8%. For Highlander, 7.5% of samples failed the genotyping and analyses was conducted without knowledge of the relationships between lambs with sires and dams. The 51% of the lambs genotyped had a sire and a dam assigned, 21% had only the sire assigned and 15% had only assigned a dam. Thus, genotyping by SNPs assigned a sire to the 72% of the lambs and a dam to the 66% of the lambs. The main problem was that only 5 of the 11 rams used as sires were genotyped and the high percentage of samples which failed the genotyping. However, taking into account the lack of information related to pedigree, we consider that a high rate of lambs had a sire and a dam assigned by the SNP panel. In conclusion, the development of a SNP panel for parentage assignment at a low price, would provide breeders with the opportunity of making mating management and control lambing easier and more relaxed while improving the known of pedigree information, ensuring high rates of genetic gain. Keywords: molecular markers, parentage exclusion, parentage verification, sheep. 650 $aMARCADORES MOLECULARES 650 $aOVINOS 653 $aMolecular markers 653 $aParentage exclusion 653 $aParentage verification 653 $aSheep 700 1 $aMACEDO, F. 700 1 $aCIAPPESONI, G.
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INIA Las Brujas (LB) |
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Registros recuperados : 34 | |
1. | | MACEDO, F.; NAVAJAS, E. Heredabilidad genómica en caracteres de canal para la raza Hereford: resultados preliminares. GGM 20 - COMUNICACIONES LIBRES - GGM. GENÓMICA Y GENÉTICA MOLECULAR In: JOURNAL OF BASIC & APPLIED GENETICS, 2016, Vol.27, Iss. 1 (Supp.). XVI LATIN AMERICAN CONGRESS OF GENETICS, IV CONGRESS OF THE URUGUAYAN SOCIETY OF GENETICS, XLIX ANNUAL MEETING OF THE GENETICS SOCIETY OF CHILE, XLV ARGENTINE CONGRESS OF GENETICS, 9-12 October 2016. PROCEEDINGS. Montevideo (Uruguay): SAG, 2016. p.264Tipo: Trabajos en Congresos/Conferencias |
Biblioteca(s): INIA Las Brujas. |
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3. | | GOLDBERG, V.; MACEDO, F.; CIAPPESONI, G. SNP genotyping for parentage identification in a Merino nucleus and in a commercial Highlander flock. Volume Electronic Poster Session - Genetic Gain - Genotyping & Phenotyping Strategies, , 359. In: Proceedings of the World Congress on Genetics Applied to Livestock Production, 11., Aotea Centre Auckland, New Zealand: WCGALP, ICAR, 11-16 feb 2018, 359.Tipo: Trabajos en Congresos/Conferencias |
Biblioteca(s): INIA Las Brujas. |
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6. | | GOLDBERG, V.; MACEDO, F.; PIERUCCIONI, F.; NAVAJAS, E.; CIAPPESONI, G. Diagnóstico para la presencia de cuernos en ovinos Merino a través del uso de herramientas genómicas. GGM 14 - COMUNICACIONES LIBRES - GGM. GENÓMICA Y GENÉTICA MOLECULAR In: JOURNAL OF BASIC & APPLIED GENETICS, 2016, Vol.27, Iss. 1 (Supp.). XVI LATIN AMERICAN CONGRESS OF GENETICS, IV CONGRESS OF THE URUGUAYAN SOCIETY OF GENETICS, XLIX ANNUAL MEETING OF THE GENETICS SOCIETY OF CHILE, XLV ARGENTINE CONGRESS OF GENETICS, 9-12 October 2016. PROCEEDINGS. Montevideo (Uruguay): SAG, 2016. p. 261Tipo: Trabajos en Congresos/Conferencias |
Biblioteca(s): INIA Las Brujas. |
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8. | | PIERUCCIONI, F.; MACEDO, F.; CORREA, O.; CIAPPESONI, G.; NAVAJAS, E. Estudio preliminar de asociación entre marcadores SNP y conteo de huevos por gramo en los ovinos criollos del Parque Nacional de San Miguel en Uruguay. [Resumen]. Congreso de la Asociación Latinoamericana de Producción Animal, 24.; Congreso de la Sociedad Chilena de Producción Animal, 40., Puerto Varas, Chile, 9 al 13 noviembre 2015. ln: Reunión ALPA (24., Puerto Varas, Chile). Resúmenes. Puerto Varas (Chile): ALPA, 2015. p. 758Tipo: Abstracts/Resúmenes |
Biblioteca(s): INIA Las Brujas. |
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12. | | CIAPPESONI, G.; GOLDBERG, V.; MACEDO, F.; ARMSTRONG, E.; GIMENO, D. Evaluaciones genéticas y uso de marcadores moleculares en ovinos. 2 - SIMPOSIOS "MEJORA GENÉTICA EN PRODUCCIÓN Y CALIDAD DE CARNE EN ESPECIES DE INTERÉS ECONÓMICO" In: JOURNAL OF BASIC & APPLIED GENETICS, 2016, Vol.27, Iss. 1 (Supp.). XVI LATIN AMERICAN CONGRESS OF GENETICS, IV CONGRESS OF THE URUGUAYAN SOCIETY OF GENETICS, XLIX ANNUAL MEETING OF THE GENETICS SOCIETY OF CHILE, XLV ARGENTINE CONGRESS OF GENETICS, 9-12 October 2016. PROCEEDINGS. Montevideo (Uruguay): SAG, 2016. p. 28Tipo: Trabajos en Congresos/Conferencias |
Biblioteca(s): INIA Las Brujas. |
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16. | | MACEDO, F.; PIERUCCIONI, F.; VILLANUEVA, B.; NAVAJAS, E. Uso de información genómica para optimizar apareamientos en ovinos criollos. [Resumen]. Congreso de la Asociación Latinoamericana de Producción Animal, 24.; Congreso de la Sociedad Chilena de Producción Animal, 40., Puerto Varas, Chile, 9 al 13 noviembre 2015. ln: Reunión ALPA (24., Puerto Varas, Chile). Resúmenes. Puerto Varas (Chile): ALPA, 2015. p. 938.Tipo: Abstracts/Resúmenes |
Biblioteca(s): INIA Las Brujas. |
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18. | | NAVAJAS, E.; MACEDO, F.; LEMA, O.M.; LUZARDO, S.; AGUILAR, I. Accuracy of genomic predictions for carcass and meat quality traits in the Uruguayan Hereford breed. Volume Species - Bovine (beef) 1, p. 636. In: Proceedings of the World Congress on Genetics Applied to Livestock Production, 11., Aotea Centre Auckland, New Zealand: WCGALP, ICAR, 11-16 feb 2018. 6 p. Acknowledgements: This work was supported by the Agencia Nacional de Investigación e Innovación (ANII) (grants RTS_1_2012_1_3489 and FMV_1_2011_1_6671), Instituto Nacional de Investigación Agropecuaria (INIA), Sociedad de Criadores de...Tipo: Trabajos en Congresos/Conferencias |
Biblioteca(s): INIA Las Brujas. |
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19. | | PRAVIA, M.I.; NAVAJAS, E.; MACEDO, F.; CLARIGET, J.M.; LUZARDO, S. Association between feed efficiency and carcass and meat quality traits in Hereford steers. Volume Electronic Poster Session - Biology - Feed Intake and Efficiency 1, p. 604. In: Proceedings of the World Congress on Genetics Applied to Livestock Production, 11., Aotea Centre Auckland, New Zealand: WCGALP, ICAR, 11-16 feb 2018. Acknowledgements: This work was supported by the Agencia Nacional de Investigación e Innovación (ANII) (grant RTS_1_2012_1_3489), Instituto Nacional de Investigación Agropecuaria (INIA), Sociedad de Criadores de Hereford (SCH), Instituto...Tipo: Trabajos en Congresos/Conferencias |
Biblioteca(s): INIA Las Brujas. |
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20. | | MACEDO, F.; CHRISTENSEN, O. F.; ASTRUC, J.M.; AGUILAR, I.; MASUDA, Y.; LEGARRA, A. Bias and accuracy of dairy sheep evaluations using BLUP and SSGBLUP with metafounders and unknown parent groups. Genetics, Selection, Evolution : GSE, 12 August 2020, Volume 52, Issue 1, Page 47. OPEN ACCESS. DOI: https://doi.org/10.1186/s12711-020-00567-1 Article history: Received 03 March 2020; Accepted 04 August 2020; Published 12 August 2020.Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA Las Brujas. |
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Registros recuperados : 34 | |
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